Recombinant:Article Title: Rejuvenation of the reconstitution potential and reversal of myeloid bias of aged HSCs upon pH treatment.
Article Snippet: .. Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6J (M. musculus) Jackson Lab B6.129P2Gpr37tm1Dgen/J BoyJ (CD45.1) mice Jackson Lab B6.SJL- Ptprca Pepcb/BoyJ Recombinant DNA - Antibodies Biotin Mouse Lineage Panel BD Biosciences Cat # 559971 Streptavidin- APC- Cy7 BD Biosciences Cat # 554063 Anti- CD11b eBioscience clone M1/70 Anti- B220 eBioscience clone RA3- 6B2 Anti- CD5 eBioscience clone 53–7.3 Anti- Gr- 1 eBioscience clone RB6- 8C5 Anti- Ter119 eBioscience 13–5921- 82 Anti- CD8a eBioscience clone 53–6.7 Anti- Sca- 1 eBioscience clone D7 Anti- c- Kit eBioscience clone 2B8 Anti- CD34 eBioscience clone RAM34 Anti- Flk2 eBioscience clone A2F10 Streptavidin, AF 700 ThermoFisher Cat # S21383 CD34- FITC, clone RAM34 eBioscience Cat # 11- 0341- 82 Flt- 3 PE eBioscience Cat # 12- 1351- 82 C- KIT–APC eBioscience Cat # 17- 1171- 82 Sca- 1 PE- Cy7 eBioscience Cat # 25- 5981- 82 cKit–APC- Cy7 eBioscience Cat # A15423 CD45.2–FITC Biolegend Cat # 109806 CD45.1–PE eBioscience Cat # 12- 0453- 82 Anti- CD3e–PE- Cy7 Biolegend Cat # 100320 Anti- LAMP2 antibody, GL2A7 Abcam Cat # ab13524 Anti LC3A/B Cell Signalling Cat # 4108 AcH3K27 Millipore Cat # 07–360 AcH4K16 Abcam Cat # ab13524 Anti- rat dylight488- conjugated antibody Jackson ImmunoResearch Cat # 112- 485- 071 Anti- Rabbit Alexa Fluor 555 Invitrogen Cat # A32794 Oligonucleotides and other sequence- based reagents - Chemicals, enzymes and other reagents 96- well round bottom plates (TPP Tissue culture plates) Midwest Scientific TP92097 96- well flat bottom plates Suspension Sarstedt Cat # 83.3924 Antifade Mounting Media VECTASHIELD Laboratories Cat # H- 1000- 10 Carboxy SNARF- 1 AM ThermoFisher Cat # C1272 Heat- Inactivated Fetal Bovine Serum GIBCO Cat# 10438- 026 DCF- DA Invitrogen Cat # D399 Click- iT Plus EdU AF488 Assay Kit Thermo Fisher Cat # C10420 SMART- Seq v4 Ultra Low Input RNA kit Clontech Catalog # 634892 Nextera XT DNA Library Preparation kit Illumina Catalog # FC- 131- 1096 (Continues) 14749726, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/acel.14324 by IN A SP - N E PA L , W iley O nline L ibrary on [13/09/2024]. ..
Sequencing:Article Title: Rejuvenation of the reconstitution potential and reversal of myeloid bias of aged HSCs upon pH treatment.
Article Snippet: .. Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6J (M. musculus) Jackson Lab B6.129P2Gpr37tm1Dgen/J BoyJ (CD45.1) mice Jackson Lab B6.SJL- Ptprca Pepcb/BoyJ Recombinant DNA - Antibodies Biotin Mouse Lineage Panel BD Biosciences Cat # 559971 Streptavidin- APC- Cy7 BD Biosciences Cat # 554063 Anti- CD11b eBioscience clone M1/70 Anti- B220 eBioscience clone RA3- 6B2 Anti- CD5 eBioscience clone 53–7.3 Anti- Gr- 1 eBioscience clone RB6- 8C5 Anti- Ter119 eBioscience 13–5921- 82 Anti- CD8a eBioscience clone 53–6.7 Anti- Sca- 1 eBioscience clone D7 Anti- c- Kit eBioscience clone 2B8 Anti- CD34 eBioscience clone RAM34 Anti- Flk2 eBioscience clone A2F10 Streptavidin, AF 700 ThermoFisher Cat # S21383 CD34- FITC, clone RAM34 eBioscience Cat # 11- 0341- 82 Flt- 3 PE eBioscience Cat # 12- 1351- 82 C- KIT–APC eBioscience Cat # 17- 1171- 82 Sca- 1 PE- Cy7 eBioscience Cat # 25- 5981- 82 cKit–APC- Cy7 eBioscience Cat # A15423 CD45.2–FITC Biolegend Cat # 109806 CD45.1–PE eBioscience Cat # 12- 0453- 82 Anti- CD3e–PE- Cy7 Biolegend Cat # 100320 Anti- LAMP2 antibody, GL2A7 Abcam Cat # ab13524 Anti LC3A/B Cell Signalling Cat # 4108 AcH3K27 Millipore Cat # 07–360 AcH4K16 Abcam Cat # ab13524 Anti- rat dylight488- conjugated antibody Jackson ImmunoResearch Cat # 112- 485- 071 Anti- Rabbit Alexa Fluor 555 Invitrogen Cat # A32794 Oligonucleotides and other sequence- based reagents - Chemicals, enzymes and other reagents 96- well round bottom plates (TPP Tissue culture plates) Midwest Scientific TP92097 96- well flat bottom plates Suspension Sarstedt Cat # 83.3924 Antifade Mounting Media VECTASHIELD Laboratories Cat # H- 1000- 10 Carboxy SNARF- 1 AM ThermoFisher Cat # C1272 Heat- Inactivated Fetal Bovine Serum GIBCO Cat# 10438- 026 DCF- DA Invitrogen Cat # D399 Click- iT Plus EdU AF488 Assay Kit Thermo Fisher Cat # C10420 SMART- Seq v4 Ultra Low Input RNA kit Clontech Catalog # 634892 Nextera XT DNA Library Preparation kit Illumina Catalog # FC- 131- 1096 (Continues) 14749726, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/acel.14324 by IN A SP - N E PA L , W iley O nline L ibrary on [13/09/2024]. ..
Suspension:Article Title: Rejuvenation of the reconstitution potential and reversal of myeloid bias of aged HSCs upon pH treatment.
Article Snippet: .. Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6J (M. musculus) Jackson Lab B6.129P2Gpr37tm1Dgen/J BoyJ (CD45.1) mice Jackson Lab B6.SJL- Ptprca Pepcb/BoyJ Recombinant DNA - Antibodies Biotin Mouse Lineage Panel BD Biosciences Cat # 559971 Streptavidin- APC- Cy7 BD Biosciences Cat # 554063 Anti- CD11b eBioscience clone M1/70 Anti- B220 eBioscience clone RA3- 6B2 Anti- CD5 eBioscience clone 53–7.3 Anti- Gr- 1 eBioscience clone RB6- 8C5 Anti- Ter119 eBioscience 13–5921- 82 Anti- CD8a eBioscience clone 53–6.7 Anti- Sca- 1 eBioscience clone D7 Anti- c- Kit eBioscience clone 2B8 Anti- CD34 eBioscience clone RAM34 Anti- Flk2 eBioscience clone A2F10 Streptavidin, AF 700 ThermoFisher Cat # S21383 CD34- FITC, clone RAM34 eBioscience Cat # 11- 0341- 82 Flt- 3 PE eBioscience Cat # 12- 1351- 82 C- KIT–APC eBioscience Cat # 17- 1171- 82 Sca- 1 PE- Cy7 eBioscience Cat # 25- 5981- 82 cKit–APC- Cy7 eBioscience Cat # A15423 CD45.2–FITC Biolegend Cat # 109806 CD45.1–PE eBioscience Cat # 12- 0453- 82 Anti- CD3e–PE- Cy7 Biolegend Cat # 100320 Anti- LAMP2 antibody, GL2A7 Abcam Cat # ab13524 Anti LC3A/B Cell Signalling Cat # 4108 AcH3K27 Millipore Cat # 07–360 AcH4K16 Abcam Cat # ab13524 Anti- rat dylight488- conjugated antibody Jackson ImmunoResearch Cat # 112- 485- 071 Anti- Rabbit Alexa Fluor 555 Invitrogen Cat # A32794 Oligonucleotides and other sequence- based reagents - Chemicals, enzymes and other reagents 96- well round bottom plates (TPP Tissue culture plates) Midwest Scientific TP92097 96- well flat bottom plates Suspension Sarstedt Cat # 83.3924 Antifade Mounting Media VECTASHIELD Laboratories Cat # H- 1000- 10 Carboxy SNARF- 1 AM ThermoFisher Cat # C1272 Heat- Inactivated Fetal Bovine Serum GIBCO Cat# 10438- 026 DCF- DA Invitrogen Cat # D399 Click- iT Plus EdU AF488 Assay Kit Thermo Fisher Cat # C10420 SMART- Seq v4 Ultra Low Input RNA kit Clontech Catalog # 634892 Nextera XT DNA Library Preparation kit Illumina Catalog # FC- 131- 1096 (Continues) 14749726, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/acel.14324 by IN A SP - N E PA L , W iley O nline L ibrary on [13/09/2024]. ..
DNA Library Preparation:Article Title: Rejuvenation of the reconstitution potential and reversal of myeloid bias of aged HSCs upon pH treatment.
Article Snippet: .. Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6J (M. musculus) Jackson Lab B6.129P2Gpr37tm1Dgen/J BoyJ (CD45.1) mice Jackson Lab B6.SJL- Ptprca Pepcb/BoyJ Recombinant DNA - Antibodies Biotin Mouse Lineage Panel BD Biosciences Cat # 559971 Streptavidin- APC- Cy7 BD Biosciences Cat # 554063 Anti- CD11b eBioscience clone M1/70 Anti- B220 eBioscience clone RA3- 6B2 Anti- CD5 eBioscience clone 53–7.3 Anti- Gr- 1 eBioscience clone RB6- 8C5 Anti- Ter119 eBioscience 13–5921- 82 Anti- CD8a eBioscience clone 53–6.7 Anti- Sca- 1 eBioscience clone D7 Anti- c- Kit eBioscience clone 2B8 Anti- CD34 eBioscience clone RAM34 Anti- Flk2 eBioscience clone A2F10 Streptavidin, AF 700 ThermoFisher Cat # S21383 CD34- FITC, clone RAM34 eBioscience Cat # 11- 0341- 82 Flt- 3 PE eBioscience Cat # 12- 1351- 82 C- KIT–APC eBioscience Cat # 17- 1171- 82 Sca- 1 PE- Cy7 eBioscience Cat # 25- 5981- 82 cKit–APC- Cy7 eBioscience Cat # A15423 CD45.2–FITC Biolegend Cat # 109806 CD45.1–PE eBioscience Cat # 12- 0453- 82 Anti- CD3e–PE- Cy7 Biolegend Cat # 100320 Anti- LAMP2 antibody, GL2A7 Abcam Cat # ab13524 Anti LC3A/B Cell Signalling Cat # 4108 AcH3K27 Millipore Cat # 07–360 AcH4K16 Abcam Cat # ab13524 Anti- rat dylight488- conjugated antibody Jackson ImmunoResearch Cat # 112- 485- 071 Anti- Rabbit Alexa Fluor 555 Invitrogen Cat # A32794 Oligonucleotides and other sequence- based reagents - Chemicals, enzymes and other reagents 96- well round bottom plates (TPP Tissue culture plates) Midwest Scientific TP92097 96- well flat bottom plates Suspension Sarstedt Cat # 83.3924 Antifade Mounting Media VECTASHIELD Laboratories Cat # H- 1000- 10 Carboxy SNARF- 1 AM ThermoFisher Cat # C1272 Heat- Inactivated Fetal Bovine Serum GIBCO Cat# 10438- 026 DCF- DA Invitrogen Cat # D399 Click- iT Plus EdU AF488 Assay Kit Thermo Fisher Cat # C10420 SMART- Seq v4 Ultra Low Input RNA kit Clontech Catalog # 634892 Nextera XT DNA Library Preparation kit Illumina Catalog # FC- 131- 1096 (Continues) 14749726, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/acel.14324 by IN A SP - N E PA L , W iley O nline L ibrary on [13/09/2024]. ..
|
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 